Part:BBa_K3721922:Design

Bacillus thuringiensis endotoxin Cry1Ab protein coding region.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Silent mutations to remove the restriction sites SpeI, EcoRI and XbaI. Adding codons TAGTGA at the end of the sequence.

Source

The endotoxin Cry1Ab (cry1Ab) gene from local isolate Bacillus thuringiensis 4A, Genbank https://www.ncbi.nlm.nih.gov/nuccore/KR612322.1

References

https://www.uniprot.org/uniprot/P0A370

https://www.ncbi.nlm.nih.gov/nuccore/KR612322.1